The structures of the cellulose nanofibers were characterized by TEM after negative staining by PTA. The samples obtained via different nanofibrillation methods and from various raw materi- als exhibit similar morphologies (Fig. 2). TEM images show the presence of very long nanofibers with varying degrees of entan- glement. The widths of single nanofibers are mainly in the range of 2–5 nm (Fig. S1). In higher plants, the diameters of single elemen- tal fibrils are reported to be ∼3 nm (Somerville et al., 2004).