3.3. Effects of KRGE on busulfan-in

3.3. Effects of KRGE on busulfan-induced decrease of male hormone
concentration and litter size
We next examined the effects of KRGE on busulfan-induced
changes in the level of the male hormone, testosterone, and litter
size, because it was reported that male sterility was one of the
adverse effects of busulfan in patients after cancer treatment. As
shown in Fig. 5A, compared with the vehicle control, treatment
with busulfan alone induced a decrease in testosterone concentration
and litter size. However, preadministration of KRGE partially
restored the busulfan-induced decrease of testosterone concentration
and litter size (Fig. 5B). These results indicate that busulfan
caused damage to the male reproductive system and that KRGE
shows preventive effects against busulfan-induced damage to the
male reproductive system.