Orthogonal array designs to optimiz

Orthogonal array designs to optimize the conditions of stage extraction of capsaicinoids and red pigments from fresh peppers, respectively.


Every extractive solution was collected and evaporated to dry at
40
C under reduced pressure with a rotary evaporator, respectively.
The residues obtained were dissolved with 20 mL solvent
and centrifuged for 10 min at 3000 rpm. Each solution was
collected and diluted with homologous solvent to a constant volume
for analysis. The solvent used was methanol for capsaicinoids
analysis, while acetone for red pigments analysis. HPLC analysis
was used for capsaicinoids, while colorimetric analysis for red
pigments.