The same procedure, excluding sequencing, was applied for transformed colonies bombarded with the PsaD–ble cassette containing constructs with appropriate primers: F-CACACCTGCCCGTCTGCCTGA, R-TGCCGTCCGTCCACTGACC for full PsaD–ble cassette and F-CCCCACTGCTACTCACAACA, R-TTAGTCCTGCTCCTCGGCCA for full 408 bp ble coding sequence (CDS) including the end of PsaD 5′ untranslated region (UTR).