2.2 Extraction of anthocyanins from

2.2 Extraction of anthocyanins from Clitoria ternatea petals
An aqueous extraction method was performed by using the different amount of the petals; 10, 20, 30 and 40 g per 100 mL of distilled water. The process started from submerging the petals in the water for 30 min. Then, both petals and water were put in the blender for 30 sec. The resulting mixture was filters through 2 layers of cheesecloths resulting in the Clitoria ternatea crude extracts. The amount of total anthocyanin content was measured and regression analysis was performed to correlate the amount of raw flowers required to achieve a specific concentration of anthocyanins. This equation would be used to adjust anthocyanin concentration in the next experiment according to the amount of raw material used during the extraction process