Shrimps were taken from the holding

Shrimps were taken from the holding tank and individually housed in a cylindrical
cage (10 cm diameter and 30 cm long) made of plastic screens (2X3 mm mesh size).
Each tank contained 20 1 of test solution and six cages, and was aerated with an air
stone. There were triplicates for each test solution with a total number of 18 juveniles
(six per replicate) for each test solution. The shrimp were also fed the commercial diet
described above.
Bioassay tests were conducted using the static renewal method with test solutions
renewed daily. Dead juveniles and uneaten feed were removed every day in the
afternoon (14:00-16:00 h) when the water was renewed. Exuviae (molted exoskeletons)
were also removed daily when they were found.