l-factorial experimentation.In this

l-factorial experimentation.
In this work, a Box–Behnken design was employed to
establish the relationship between the reaction variables and the
deproteinization degree. Furthermore, the ridge analysis has been
employed to optimize the experimental conditions permitting the
higher deproteinization degree. The purity level of chitin was followed by the evaluation ofthe mineral and protein contents. Chitin
was then converted to chitosan by chemical deacetylation. The
antibacterial activity of the acid-soluble chitosan of shrimp waste
was investigated.