Venous blood was collected in the h

Venous blood was collected in the heparinized tubes
from both normal and occupational sprayers as coded
samples from both the control and sprayers. Samples
were transported to the analytical toxicology laboratory
in ice-cold condition immediately after the collection.
The content was centrifuged at 1000g for 10 min for
RBC separation. The buffy coat was removed and the
remaining RBCs were drawn from the bottom and then
the packed RBCs were washed three times with cold
phosphate-buffered saline (pH 7.4). After the final
wash, the RBCs were lysed by hypotonic shock and
different dilutions were used as hemolysate.21 The
analyses of different biochemical indices were carried
out in the hemolysate on the same day.