2.6. Biopsy and bacterial identific

2.6. Biopsy and bacterial identification
One day after the final administration, the mice were sacrificed and their gastric mucosa were biopsied for the detection of H. pylori. The biopsy samples (3
3 cm) from the gastric pylorus were 58 D. Park et al. / Regulatory Toxicology and Pharmacology 76 (2016) 57e62 minced and applied to CLO kits, then incubated at 35 C for 24 h to examine urease activity. The reaction (color change) was determined as negative for bright yellow, false (partially) positive for thick yellow, or positive for thick (dark) red.