Raw materials were dried and ground

Raw materials were dried and ground into powder. The powder (100 g) was heated in a water bath twice with 650 mL of NaOH (0.1 mol/L) and 650 mL of 90% ethanol at 45
C, and the heating time was 2 hours each time. Then the mixture was centrifuged at 4000 rpm for 10 minutes. The supernatant containing the crude protein was combined and doubled using distilled water. The pH was adjusted to 6.3 to make the protein precipitate. The precipitate of protein was lyophilized