Folin–Ciocalteu reagent is mixture

Folin–Ciocalteu reagent is mixture of phosphomolybdate and phosphotungstate.

Phenolic compound cause the reduction of phosphotungstate-phosphomolybdate complex to blue reaction products in alkaline conditions and the estimation of phenolics involves the measurement of the absorbance of this colored complex at 765 nm.

It measures the amount of the substance need to inhibit the oxidation of the reagent.

The total phenolic content was expressed in terms of % Gallic acid. Results were reported in Table 1.