The entire set of 702 sugarcane mic

The entire set of 702 sugarcane microsatellite markers
designed and developed at National Research Centre on Plant
Biotechnology, IARI, New Delhi, were evaluated on high and
low sugar lines of sugarcane germplasm and segregating
populations of high and low sugar lines with their parents for
utility in molecular characterization of elite sugarcane
genotypes for tagging and mapping of sugar and sugar related
traits. The evaluation was based on polyacrylamide gel
electrophoresis / UV- Visualization ethidium bromide stained
SSR allele’s detection system. One hundred sixty eight
microsatellites markers (26% of the total ) were found to be
highly robust and produced a total of 977 polymorphic loci, of
which 78 loci were always coming from low sugar parent and
130 loci were coming from high sugar parents, which is high
sugar commercial cultivar. These newly developed
microsatellites markers will be useful in sugarcane germplasm
evaluation, variety identity tests, cross fidelity assessments
and polycross paternity analysis.