Glucoamylase (EC 3.2.1.3), an indus

Glucoamylase (EC 3.2.1.3), an industrial enzyme, hydrolyses α-(1→4) and α-(1→6) glucosidic bonds of starch from the non-reducing ends successively to produce glucose and is used for the production of glucose from starch. The enzyme has been immobilized using different methods including alginate entrapment.Alginate immobilization is widely used for microorganisms but it requires an additional process for the immobilization of enzymes due to the fact that even large enzymes with molecular weights of 300,000 leak out of alginate beads.One method of enzyme immobilization in alginate beads is to covalently link the enzyme to alginate before the bead formation [6]. Glucoamylase and Saccharomyces cerevisiae were co-immobilized within alginate matrix to which glucoamylase was covalently linked through cyanuric chloride method to prevent the leakage of the enzyme out of alginate beads