2.2. Spathe browningThe browning of

2.2. Spathe browning
The browning of anthurium cut
flowers spathe was assessed
using two methods. The
first method of browning assessment was
by visualizing the total brown area on the spathes of 10 individual
flowers using a scale from 1 to 5; 1 = no chilling injury; 2 = mild
injury (1–20% of spathe affected); 3 = moderate injury (21–50% of
spathe affected); 4 = severe injury (51–80% of spathe affected);
5 = very severe injury (81–100% of spathe affected). CI was
calculated as P (number on CI scale

number of
flowers at that
number on the CI scale)/total number of
flower in each group,
according to Promyou et al. (2012). As a second method for
assessment of spathe browning, surface color of spathe was
measured with a Minolta spectrophotometer (CR-400) using CIE
color parameters L* (light/dark), a* (red/green) and b*(yellow/blue)
values and browning index calculated by formula as follow:
Browning Index (BI) = [100 (x

0.31)]/0.17, where x = (a* + 1.75L*)/
(5.645L* + a* – 0.3012b*), according to Ding and Ling (2014).
2.3. Electrolyte leakage and MDA content
Ten discs, 2 mm thickness and 15 mm diameter, were excised
from the spathe with a cork borer, rinsed with 50 mL of distilled