After the quantitative analysis of the enzyme activity and
the qualitative analysis of the oligosaccharide production (Secondary
screening), four strains showing potential for FOS
production were selected. After the qualitative analysis of
oligosaccharide production, a synthesis reaction was carried out
to determine the FOS content. All the enzymes studied produced
fructo-oligosaccharide 1-kestose (GF2). On the other hand, the
nystose (GF3) and fructofuranosyl-nystose (GF4) contents were
only important in the syntheses performed by the LEB-V10 and
LEB-U5 enzymes.
The genera of these microorganisms were, in sequence, Candida
(LEB-I3 strain), Rhodotorula (LEB-U5 and LEB-V10
strains) and Cryptococcus (LEB-V2 strain).