To understand the enzyme hydrolysis performance under
different conditions, the percentage of glucan and xylan
solublized (glucan and xylan conversion) was calculated. For
S. cerevisiae RWB222, the average glucan conversion to
monomer sugar or ethanol was 93%, and the average xylan
conversion to monomer sugar or ethanol was 95% at 378C.
For Z. mobilis 8b, the average glucan and xylan conversion
to monomer sugar or ethanol was much lower at 378C,
presumably due to cellulase inhibition by the high concentration
of residual glucose and xylose remaining
(Fig. 1B). Conversions of glucan and xylan at 308C were
similar for both Z. mobilis 8b and S. cerevisiae RWB222, at
almost 90%. SSCF is a hydrolysis dominated process if the
residual glucose and xylose are at very low concentrations in
the broth, consistent with the similar enzymatic hydrolysis
yield of cellulose and hemicellulose achieved by both
organisms at 308C as shown in Table I.