Fatty acids analysisThe saturated,

Fatty acids analysis
The saturated, monounsaturated, polyunsaturated and transfatty
acids were determined using Gas Chromatography (GC)
coupled with Flame Ionization Detector (FID) (AOAC,
2005). Triglycerides and phospholipids were first converted into
Fatty Acid Methyl Esters (FAME) by saponification and esterification
in the presence of Boron Trifluoride. The resulting substance
was then analyzed using GC coupled with FID (Agilent
Technologies, USA). The GC with FID was equipped with DBWAX
capillary column (30 m · 0.25 mm, film thickness
0.25 lm). Helium was chosen as the carrier gas. The total fat
content was calculated as a sum of individual fatty acids. The
saturated, monounsaturated, polyunsaturated and trans fatty
acids were calculated as the sum of the respective fatty acids