Soil and plant tissues samples were collected monthly and
transported to the laboratory for the determination of total Hg concentration. Plants were initially separated into roots, stems and
leaves. Afterwards, they were washed three times with sterile
water and stored in paper bags for three-day drying at 40 Cina
Binder forced convection oven with a capacity of 115 L. Next, bio-
mass accumulation was determined by weighing the samples
using a digital balance OHAUS Adventurer. The room conditions
were maintained with a temperature of 27–30 C and relative
humidity between 55% and 65%.