and anticancer properties [12]. The present study for the
first time establishes andrographolide as the major bioactive
anti-malarial constituent of the plant A. paniculata using
both in vitro and in vivo approaches. The encouraging in
vitro antiplasmodial activities of andrographolide prompted
us to evaluate the interaction of andrographolide (AND)
with other established anti-malarial compounds such as
curcumin (CUR) and artesunate (AS), the hemisuccinate
derivative of artemisinin in vitro on Plasmodium falciparum,
and in vivo on Plasmodium berghei ANKA strains. This adds
new information on combination of potent anti-malarial
compounds for novel combinatorial drug therapy against
drug-resistant malaria.