The Listeria monocytogenes strain o

The Listeria monocytogenes strain obtained from Microbial Type Culture Collection (MTCC), Chandigarh was used
as known positive strain in PCR analysis. 1.5 ml of enriched broths was taken into eppendorf tubes and bacteria
were pelleted by centrifugation at 6000 rpm for 5 min. To the pellet 50 μl of molecular grade water was added and
incubated at 1000C for 10 min. and snap chilled to release DNA. Then centrifuged at 13000 rpm for 5 min and the
supernatants were used in PCR for DNA amplification.